The growth of ordered two-dimensional sheets of ribosomal particles from salt-alcohol mixtures.
نویسندگان
چکیده
A procedure for the in vitro growth of well-ordered two-dimensional sheets from ribosomal particles using salts and salt-alcohol mixtures has been developed. Employing this procedure, ordered two-dimensional sheets of the wild type as well as of mutated 50 S ribosomal subunits from Bacillus stearothermophilus can readily be obtained. These sheets, stained with uranyl acetate or gold-thioglucose, are suitable for three-dimensional image reconstruction. They consist of relatively small unit cells with dimensions of 160 +/- 15 and 365 +/- 20 A. Diffraction patterns of electron micrographs of these sheets contain features to 25 A resolution.
منابع مشابه
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عنوان ژورنال:
- Analytical biochemistry
دوره 167 1 شماره
صفحات -
تاریخ انتشار 1987